Laboratory Manual for Practical Biochemistry (Record no. 33242)

MARC details
000 -LEADER
fixed length control field 08812nam a22002057a 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9789350902769
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 612.05 SHA-L
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Shivaraja, Shankara
245 ## - TITLE STATEMENT
Title Laboratory Manual for Practical Biochemistry
250 ## - EDITION STATEMENT
Edition statement 2nd
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc New Delhi
Name of publisher, distributor, etc Jaypee Brother
Date of publication, distribution, etc 2013
300 ## - PHYSICAL DESCRIPTION
Extent XVI,164p.
500 ## - GENERAL NOTE
General note Contents<br/>Section 1: Basic Laboratory Principles<br/>1. Laboratory Equipments, Glassware, Laboratory Hazards and<br/> General Laboratory Rules......................................................................................3<br/>Laboratory Equipments 3<br/>Laboratory Glassware 4<br/>Laboratory Hazards 7<br/>Laboratory Safety Procedure 7<br/>Laboratory First Aid 10<br/>2. Laboratory Units, Basic Calculations and Preparation of Solutions ...............11<br/>Units 11<br/>Preparation of Molar and Normal Solutions 13<br/>Preparation of Percent Solutions 14<br/>3. Acids, Bases, Alkalies, pH, Indicators and Buffers ...........................................15<br/>Acids and Bases 15<br/>Alkali 15<br/>Indicators 15<br/>Buffers 16<br/>Section 2: Qualitative Experiments<br/>4. Carbohydrates .................................................................................<br/>Laboratory Manual for Practical Biochemistry<br/>xii<br/>6. Proteins ..................................................................................................................30<br/>Definition 30<br/>Elemental Composition 30<br/>Functions 30<br/>Classification 30<br/>7. Color Reactions of Proteins ..................................................................................32<br/>Protein and its Color Reactions 32<br/>8. Precipitation Reactions of Proteins .....................................................................38<br/>Methods of Precipitation Reactions 38<br/>Significance of the Precipitation Reactions 39<br/>Scheme for Identification of Unknown Proteins 41<br/>9. Reactions of Nonprotein Nitrogenous (NPN) Substances ..................................42<br/>Nonprotein Nitrogenous Substances 42<br/> 10. Scheme for Identification of Physiologically Important Substances ................44<br/>11. Urine Analysis (Normal Urine) ............................................................................45<br/>Analysis of Normal Constituents in Urine 45<br/>Determination of Specific Gravity 45<br/>12. Analysis of Abnormal Constituents in Urine ......................................................50<br/>Physical Characteristics 50<br/>Chemical Constituents 50<br/>13. Inborn Errors of Metabolism ...............................................................................54<br/>Screening Tests for Inborn Errors of Amino Acid Metabolism 54<br/>Screening Tests for Inborn Errors of Carbohydrate Metabolism 56<br/>14. Analysis of Gastric Juice ......................................................................................58<br/>Tests for Normal Constituents 58<br/>Tests for Abnormal Constituents 59<br/>15. Analysis of Saliva ..................................................................................................61<br/>Collection of Saliva 61<br/>16. Preparation of Hemin Crystals ............................................................................63<br/>Preparation of Hemin Crystals 63<br/>17. Sample Collection and Preservation ...................................................................64<br/>Blood Sample Collection and Preservation 64<br/>Urine Collection and Preservation 65<br/>xiii<br/>Contents<br/>Section 3: Quantitative Experiments<br/>18. Estimation of Blood Sugar by Folin-Wu Method ...............................................69<br/>Principle 69<br/>Procedure 69<br/>Calculation 70<br/>Clinical Aignificance 71<br/>Other Methods 71<br/>19. Estimation of Blood Glucose by O-Toluidine Method .......................................72<br/>Principle 72<br/>Procedure 72<br/>Calculation 73<br/>20. Estimation of Blood Urea by Diacetyl Monoxime (DAM) Method ..................74<br/>Principle 74<br/>Procedure 74<br/>Calculation 75<br/>Clinical Significance 75<br/>Other Methods 76<br/>21. Estimation of Serum Total Protiens, Albumin and Albumin/Globulin Ratio<br/>by Biuret Method ..................................................................................................77<br/>Principle 77<br/>Procedure 77<br/>Calculation 78<br/>Clinical Significance 78<br/>22. Estimation of Serum Albumin by Dye-Binding Method ..................................80<br/>Principle 80<br/>Procedure 80<br/>Calculation 80<br/>Other Methods 81<br/>23. Estimation of Serum Inorganic Phosphate by Fiske and<br/> Subba Row Method ...............................................................................................82<br/>Functions of Phosphorus 82<br/>Principle 82<br/>Procedure 83<br/>Calculation 83<br/>Clinical Significance 83<br/>Other Method 84<br/>Laboratory Manual for Practical Biochemistry<br/>xiv<br/>24. Estimation of Serum Cholesterol by Zak’s Method ...........................................85<br/>Principle 85<br/>Procedure 85<br/>Calculation 86<br/>Clinical Mignificance 86<br/>Other Methods 86<br/>25. Estimation of Serum Uric Acid by Caraway Method .......................................87<br/>Principle 87<br/>Procedure 87<br/>Calculation 88<br/>Clinical Significance 88<br/>Other Methods 88<br/>26. Estimation of Serum Bilirubin by Malloy and Evelyn Method ........................89<br/>Principle 89<br/>Procedure 89<br/>Calculation 90<br/>Clinical Significance 90<br/>27. Estimation of Serum Creatinine by Jaffe’s Alkaline Picrate Method ..............92<br/>Creatine 92<br/>Principle 92<br/>Calculation 93<br/>Clinical Significance 93<br/>28. Estimation of Vitamin C (Ascorbic Acid) in Plasma by<br/> Sauberlich Method ................................................................................................94<br/>Principle 94<br/>Procedure 94<br/>Calculation 94<br/>Clinical Sgnificance 95<br/>29. Estimation of Vitamin A in Serum by Bessey Method ......................................96<br/>Principle 96<br/>Procedure 96<br/>Calculation 96<br/>Clinical Significance 96<br/>30. Estimation of Serum Transaminases by Reitman and Frankel Method ..........97<br/>Principle 97<br/>Procedure 97<br/>Calculation 98<br/>Clinical Significance 98<br/>Other Methods 99<br/>xv<br/>Contents<br/>31. Estimation of Serum Alkaline Phosphatase by<br/> Kind and King Method .......................................................................................100<br/>Principle 100<br/>Clinical Significance 101<br/>32. Estimation of Serum Calcium by O-Cresolphthalein<br/> Complexone Method ...........................................................................................102<br/>Principle 102<br/>Calculation 103<br/>Clinical Significance 103<br/>Other Methods 103<br/>33. Estimation of Urine Creatinine By Jaffe Alkaline Picrate Method ................104<br/>Principle 104<br/>Procedure 104<br/>Calculation 104<br/>Clinical Significance 105<br/>34. Estimation of Urinary Proteins by Turbidimetric Method .............................106<br/>Principle 106<br/>Procedure 106<br/>Calculation 106<br/>Other Methods 107<br/>Section 4: Clinical Biochemistry Techniques<br/>and Recent Aspects of Clinical<br/>Biochemistry<br/>35. Colorimetry—General Principle and Procedure .............................................111<br/>Principle 111<br/>Procedure 113<br/>Calculation 113<br/>Applications of Colorimetric Assays 113<br/>36. Potentiometry ......................................................................................................114<br/>Indicator Electrodes 115<br/>pH Meter 115<br/>Ion-Selective Electrodes 117<br/>37. Flame Photometer—General Principle and Procedure ...................................118<br/>Flame Photometry 118<br/>Laboratory Manual for Practical Biochemistry<br/>xvi<br/>38. Electrophoresis—General Principle and Procedure ........................................121<br/>Electrophoresis 121<br/>39. Chromatography—General Principle and Procedure.....................................124<br/>Chromatography 124<br/>40. Automation in Clinical Biochemistry ................................................................128<br/>Types of Analyzers 128<br/>41. Quality Control in Clinical Biochemistry .........................................................130<br/>Investigation of Clinical Laboratory 130<br/>42. Oral Glucose Tolerance Test...............................................................................134<br/>Assessing the Status of Glucose Tolerance 134<br/>43. Case Reports ........................................................................................................139<br/>44. Normal Values of Biochemical Parameters ......................................................147<br/>Section 5: Reagent Preparations<br/>45. Reagent Preparations .........................................................................................153<br/>Reagents for Qualitative Experiments 153<br/>Reagents for Quantitative Experiments 155<br/>Index 159
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Human physiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Shivaraja, Shankara
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://books.google.co.in/books?id=ZjbrmQEACAAJ&dq=Laboratory+Manual+for+Practical+Biochemistry+By:+Shivaraja,+Shankara.&hl=en&sa=X&ved=0ahUKEwjJt5-n7o3iAhXS63MBHY0RDnQQ6AEIKjAA">https://books.google.co.in/books?id=ZjbrmQEACAAJ&dq=Laboratory+Manual+for+Practical+Biochemistry+By:+Shivaraja,+Shankara.&hl=en&sa=X&ved=0ahUKEwjJt5-n7o3iAhXS63MBHY0RDnQQ6AEIKjAA</a>
901 ## - LOCAL DATA ELEMENT A, LDA (RLIN)
Acc. No. 22280
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Books
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Source of acquisition Cost, normal purchase price Inventory number Total Checkouts Full call number Barcode Date last seen Date last borrowed Price effective from Koha item type Collection code Uniform Resource Identifier
    Dewey Decimal Classification     Amity Central Library Amity Central Library AIB 30/09/2015 SBA 195.00 SBA / 10952 07/08/2015 1 612.015 SHA-L 22280 31/08/2017 22/08/2017 30/09/2015 Books    
    Dewey Decimal Classification   Not For Loan Amity Central Library Amity Central Library AIB 30/09/2015 SBA 195.00 SBA / 10952 07/08/2015   612.015 SHA-L 22281 30/09/2015   30/09/2015 Reference Book Reference https://epgp.inflibnet.ac.in/UploadedContent.php
    Dewey Decimal Classification     Amity Central Library Amity Central Library AIB 30/09/2015 SBA 195.00 SBA / 10952 07/08/2015 2 612.015 SHA-L 22282 07/03/2018 18/10/2016 30/09/2015 Books    
    Dewey Decimal Classification     Amity Central Library Amity Central Library AIB 30/09/2015 SBA 195.00 SBA / 10952 07/08/2015 2 612.015 SHA-L 22283 21/11/2017 17/03/2017 30/09/2015 Books    
    Dewey Decimal Classification     Amity Central Library Amity Central Library AIB 30/09/2015 SBA 195.00 SBA / 10952 07/08/2015 1 612.015 SHA-L 22284 15/02/2018 30/01/2018 30/09/2015 Books    
Web Counter

Powered by Koha